Lateral Flow Immunoassay As Novel Screening Tool For Potential Cross-Reacting Protein In Crude Plant Latex As Tested For Plasmodium, Dengue, HBV, HCV, HIV

In vitro diagnostic kits use immune chromatographic assay with gold nanoparticle conjugated antigen / antibody coated in the kit that specifically detects of pathogen or to a blood serum tested. Such are available for wide variety diseases and different could antigens targeted. this research article I present novel finding when latex Calotropis gigantea Thevetia peruviana is used as sample targeting Histidine rich protein Plasmodium falciparum, displayed positive reaction which indicates homologous exists these two plants falciparum. Similarly, it was noticed LDH P. vivax gigantea. Further gigantea, Tabernaemontana divaricate had activity against HCV, Dengue, HIV. The Carica Papaya HCV. Hence brings out concept human specific lateral flow screening plant sap identify potential cross-reacting proteins even layman can screen doesn’t require expertise like docking studies. pave way LFA medicinal interest extracts detecting antigens, antibodies sera particular cross react produce agglutination.